The isolation and characterization of proteus mirabilis from. Clinical relevance of proteus mirabilis in hospital patients. Proteus mirabilis is a gramnegative, facultatively anaerobic, rodshaped bacterium. It can be found in soil, water, and the intestinal tract of mammals, including humans. Microbiological analysis of drinking water quality of ananthanar. Proteus mirabilis swarming behavior is characterized by the development of concentric rings of growth that are formed as cyclic events of swarmer cell differentiation, swarming migration, and cellular differentiation are repeated during colony translocation across a surface. The genus proteus currently consists of five named species. Proteus mirabilis is a gramnegative and anaerobic bacterium, which upon laboratory analysis demonstrates swarming motility coordinated translocation of the bacterial population across solid surfaces and urease activity.
These infections are often caused by escherichia coli and proteus mirabilis. The detection of proteus mirabilis genes by pcr technique for positive. Cre bacteria have been associated with the use of medical devices such as. Antimicrobial resistance and genotype analysis of extended. Proteus mirabilis isolated from urinary tract infections in some hospitals of baghdad city. Proteus mirabilis proteus mirabilis is part of the enterobacteriaceae family. Microbiological analysis of drinking water quality of. Individuals suffering from urinary tract infections caused by proteus mirabilis often.
The isolation and characterisation of proteus mirabilis. The isolation and characterisation of proteus mirabilis bacteriophages and their effect on the colonisation and blockage of urinary catheters. Proteus mirabilis definition of proteus mirabilis by. Urinary tract infections utis are a severe public health problem and are caused by a range of pathogens, but most commonly by escherichia coli, klebsiella pneumoniae, proteus mirabilis, enterococcus faecalis and staphylococcus saprophyticus. Proteus mirabilis the genus proteus, according to bergeys manual of determinative bacteriology, is a group of straight rod gram negative bacteria around 0. Characterization of a multiple antibiotic resistance plasmid from proteus mirabilis camille talandafath loyola university chicago this thesis is brought to you for free and open access by the theses and dissertations at loyola ecommons. Elucidating the genetic basis for catheter blockage and encrustation in proteus mirabilis nina holling a thesis submitted in partial fulfilment of the requirements of.
Citrobacter freundii and proteus mirabilis are reported. The infections caused by proteus mirabilis and pseudomonas aeruginosa. All produced ornithine decarboxylase and were susceptible to members of the penicillincephalosporin groups of antibiotics. Proteus mirabilis is an enteric organism and subsequent analysis showed that bacteria isolated from faecal material and catheter biofilms of the same patient were identical, indicating that catheter encrustation of longterm catheterised patients occurs from p. Clinical relevance of proteus mirabilis in hospital. Clinically, this organism is most frequently a pathogen of the urinary tract, particularly in patients undergoing longterm catheterization. Muestra aglutinacion, motilidad, y actividad ureasa. The isolation and characterization of proteus mirabilis from different. Characterization of a multiple antibiotic resistance plasmid. Proteus mirabilis microbiology a gramnegative pathogen linked to utis, wound infections habitat p mirabilis may be found in water, soil, feces proteus mirabilis proteus mirabilis. Catheterassociated urinary tract infections cautis represent the most common type of nosocomial infection and are a major health concern due to the complications and frequent recurrence. Proteus mirabilis undergoes swarming differentiation at much higher concentrations of agar 1. Proteus mirabilis compromises the care of many patients undergoing longterm indwelling bladder catheterization.
Enimie endurance oaikhena 1, dominic bawa makaije 1, samuel dangmwan denwe 1, muhammad muktar namadi 2, al i ahmed haroun 1. The infections associated with biomaterials are crucial therapeutic obstacles, due to the bactericidal resistance of the biofilm. Proteus mirabilis is normally found in the human intestine with other organisms composing a highly complex in microflora. Pdf one hundred different clinical specimens were collected from 100 patients who were referred and admitted to hilla teaching hospital during a. High recurrence rates and increasing antimicrobial resistance among uropathogens threaten to greatly increase the economic burden of these infections. Investigation of antioxidant activities of fruit juices and herbal teas and their antimicrobial effects on proteus mirabilis submitted by ye sim kumbet in partial fulfillment of the requirements for the degree of master of science in biochemistry department, middle east technical university by. Urinary tract infections are primarily caused by gramnegative bacteria, but grampositive pathogens may also be involved. This indicates a higher resolving strain differentiation effect of the proteus mirabilis and proteus morganii. It is a small gramnegative bacillus and a facultative anaerobe. Proteus mirabilis in vitro biofilms proteus has been shown to produce biofilms in diverse environments from aquatic conditions6 to indwelling devices vascular access portshemasites, scleral buckles, ureteral stents, urethral catheters and tracheesophageal voice prostheses provox2. Biochemical characterization and antimicrobial susceptibility. Proteus mirabilis in sim medium proteus mirabilis is indole negative kovacs reagent did not turn red and hydrogen sulfide positive agar turned black.
Studies on the epidemiology and pathogenesis of proteus mirabilis infections of the urinary tract in patients with urostomies. Proteus mirabilis is characterized by its swarming motility, urease activity, its ability to ferment maltose and its inability to ferment lactose. Proteus is a genus of gramnegative bacteria belonging to the family of enterobactericeae. Proteus mirabilis charakterystyka ogolna proteus mirabilis jest gramujemna, prototroficzna, urzesiona perytrychalnie i nieprzetrwalnikujaca krotka paleczka o dlugosci 12 m, nalezaca do rodziny ente or acei braceaet. Proteus mirabilis symptoms, causes, diagnosis, treatment. Molecular study of proteus mirabilis bacteria isolated from urine and. Thirteen indoleproducing, swarming strains of proteus were identified by additional biochemical testing as being proteus mirabilis. Proteus vulgaris is a rod shaped gramnegative chemoheterotrophic bacterium. Aug 15, 2019 biochemical test and identification of proteus mirabilis. Isolation and characterization of proteus mirabilis and escherichia coli bacteriophages by ira aryani binti wirjon thesis submitted in fulfillment of the requirements. First, a gram stain was done to determine if the unknown was gram negative or gram positive. Characterization of indolepositive proteus mirabilis.
Studies on the epidemiology and pathogenesis of proteus. In professional wrestling, british company progress wrestling has announced the introduction of a proteus championship. Biochemical identification and characterization of dna. Isolation and characterization of proteus mirabilis. Multidrug resistant proteus mirabilis isolated from urinary tract. In this lesson, learn about the causes, symptoms, and treatments for proteus. Individuals suffering from urinary tract infections caused by proteus mirabilis. Proteus mirabilis is a gramnegative, facultatively anaerobic, rod shaped bacterium. Carbapenemresistant enterobacteriaceae cre infections come from bacteria normally found in a healthy persons digestive tract. Proteus mirabilis, an organism that is often considered to be implicated in contamination and colonization, is occasionally isolated in severe infections. Apr 02, 2016 bioremediation potentials of heavy metal tolerant bacteria isolated from petroleum refinery effluent. Mar 30, 2016 studies on the epidemiology and pathogenesis of proteus mirabilis infections of the urinary tract in patients with urostomies. Pdf isolation of proteus mirabilis and proteus vulgaris. Not forgotten, my appreciation to all technicians and office staff of the school of biological sciences.
Trends in the susceptibilities of proteus mirabilis. Currently, the genus proteus consists of five species. Proteus mirabilis is also the cause of urinary infections. Distribution of uropathogens may differ by type of infection or patient population table 12.
Complicated catheterassociated urinary tract infections. Proteus mirabilis is a gramnegative bacterium which is wellknown for its ability to robustly swarm across surfaces in a striking bullseye pattern. Isolation of proteus mirabilis and proteus vulgaris from different clinical sources and study of some virulence factors article pdf available january 20 with 2,085 reads how we measure reads. Proteus mirabilis is one of these and is most referenced in its tendency to produce staghorn calculi composed of struvite magnesium ammonium phosphate that fill the human renal pelvis. Roger, on the other hand, admitting that milk sugar is not fermented, notes that when the casein is precipitated the. Gramnegative bacterial species that cause cautis express a number of virulence factors associated with adhesion, motility. Proteus mirabilis, a gramnegative rodshaped bacterium most noted for its swarming motility and urease activity, frequently causes catheterassociated urinary tract infections cautis that are often polymicrobial. Biochemical identification and characterization of dna groups. Antimicrobial susceptibilities of proteus mirabilis. It has been found that most esbls were derivatives of tem1 type, tem2 type and shv1 type of. Proteus mirabilis is part of the normal flora of the human gastrointestinal tract. Proteus mirabilis on blood agar for most strains of p. The aim of this study was to assess the susceptibility of p. In addition, your digestive system is a home of many microorganisms.
Pdf multidrug resistant proteus mirabilis isolated from urinary. Proteus mirabilis can adhere to the medical equipment such as urinary catheters and enter your body where they travel and reach your urinary system and cause an infection. The effect of these phages, either individually or as a cocktail, on p. Bioremediation potentials of heavy metal tolerant bacteria. L of antibiotic were added to each well coated with the biofilm. Molecular epidemiology of proteus mirabilis infections of. Sc thesis, college of medicine, university of babylon. Biochemical test and identification of proteus mirabilis. Proteus mirabilis, a gramnegative rodshaped bacterium most noted for its swarming motility and urease activity, frequently causes catheterassociated urinary tract infections cautis that are. Proteus mirabilis belongs to the enterobacteriaceae family with the features of swarming motility and production of urease to generate ammonia 1, 2. Thesis submitted in fulfillment of the requirements for the degree of. Proteus mirabilis can migrate across the surface of solid media or devices using a type of cooperative group motility called swarming.
Proteus mirabilis was first discovered by a german pathologist named gustav hauser williams and schwarzhoff, 1978. Biochemical characterization and antimicrobial susceptibility trends of. Rods of the proteus genus are commonly isolated from patients, especially from the urinary tracts of the catheterised patients. Proteus mirabilis es una bacteria gram negativa, facultativamente anaerobico. Proteus mirabilis is most frequently associated with infections of the urinary tract, especially in complicated or catheterassociated urinary tract infections.
Apr 29, 20 rods of the proteus genus are commonly isolated from patients, especially from the urinary tracts of the catheterised patients. Biochemical characterization and antimicrobial susceptibility trends of proteus mirabilis isolated from patients suspected with urinary tract infections attending sickbay hospital, zaria, kaduna, nigeria. Pdf abstract a total of 15 isolates of proteus out of 100 samples comprising of. The 12 and 24hour biofilms were formed according to the given methodology. When this organism, however, enters the urinary tract, wounds, or the lungs it can become pathogenic. On the basis of the results of these five tests, 49% of the isolates fell into dna groups 5 and 6, 37% fell into dna group 2. Proteus mirabilis biofilms and catheterassociated urinary. Proteus mirabilis an overview sciencedirect topics. Pdf isolation of proteus mirabilis and proteus vulgaris from.
Pdf bacteriological and molecular study of some isolates of. Isolation and characterization of proteus mirabilis and. The ajumalis coding of proteus mirabilis and proteus morganii table 5 and 6 presented different antibiogram type, making them phenotypically different from one another, even though they are of the same species. It can also be found free living in water and soil. It can be found as part of the micro flora in the human intestine. Characterization of proteus mirabilisprecocious swarming. Studies on the epidemiology and pathogenesis of proteus mirabilis. Elucidating the genetic basis for catheter blockage and. Because of the presence of perspicuous flagella, species included this genus are motile and most strains swarm in periodic cycles, producing a characteristic concentric swarming pattern, or spread in a uniform film over uterine media solidified with agar or gelatin, which is evident in the streak plates done over the course of a few weeks, the streak plates showing the. In vivo, swarmer cells have been demonstrated in mouse models of ascending urinary tract infection. I am sincerely grateful to the institute of postgraduate studies staff who have.
Essay about the proteus vulgaris bacterium 673 words. They also inhabit other external environments, and are specially prevented in hospitals and healthcare. In recent years, in addition to the temtype esbls, there. It turned out to be a gram negative organism, so further tests were ordered based on this fact.
These strains were characterized by 40 biochemical tests and by susceptibility testing to 11 antibiotics. Proteus species are distinguishable from most other genera by their ability to swarm across an agar surface jacobsen et al. In addition to being a leading cause of urinary tract infections uti, p. The secreted hemolysins of proteus mirabilis, proteus vulgaris, and morganella morganii are genetically related to each other and to the alphahemolysin of escherichia coli. Proteus infectious disease and antimicrobial agents.
After removing the medium containing the planktonic forms, 100. Proteus mirabilis is a type of bacteria that can cause infections in the body, particularly urinary tract infections. Proteus mirabilis belongs to the enterobacteriaceae family with the features of swarming motility and production of urease to generate ammonia 1,2. Proteus ranks third as the cause of hospitalacquired infections stamm, 1999 three species.
This cycle produces the bullseye colony often associated with cultures of p. Szczegolnym przejawem zdolnosci do lps lipopolisacharyd. Proteus is widespread in the environment and makes up part of the normal flora of the human gastrointestinal tract. Essay about the proteus vulgaris bacterium 673 words bartleby.
These infections may be accompanied by urolithiasis, the development of bladder or kidney stones due to alkalinization of urine from ureasecatalyzed urea hydrolysis. Assessment the impact of tested antibiotics on proteus mirabilis biofilm. Sir, the incidence of resistance to fluoroquinolones among aerobic gramnegative bacilli agnb has increased during the last decade. The isolation and characterization of proteus mirabilis. Estimation of production of biofilm by proteus mirabilis bacilli. We placed 43 isolates belonging to the proteus vulgaris complex into proposed dna groups genomovars using five previously recommended tests tests for esculin hydrolysis, production of acid from salicin, lrhamnose fermentation, and elaboration of dnase and lipase.
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